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Ouchterlony Double Diffusion is a classical immunological technique used to study antigen-antibody interactions in vitro. It is based on the principle of passive diffusion of antigens and antibodies through a semisolid medium, usually agar or agarose, resulting in the formation of visible precipitin lines where optimal antigen-antibody binding occurs. The method was developed by Örjan Ouchterlony in the mid-20th century and remains _________ _____ ___ ________ ______ ____ _________ ___ ___ _____.
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