Illustrate the application of genetic engineering in medicine, focusing on the production of recombinant human insulin.

Genetic engineering has revolutionized medicine by enabling the production of essential therapeutic proteins, including recombinant human insulin. This breakthrough has provided a reliable and scalable solution for diabetes management, replacing earlier methods that relied on insulin extracted from animals. The application of genetic engineering in producing human insulin involves several key steps and technologies that ensure high efficiency and safety.  

The process begins with the identification and isolation of the human insulin gene. Scientists extract the gene responsible for insulin production from human DNA and modify it for expression in bacterial or yeast cells. To achieve this, recombinant DNA technology is employed, wherein the insulin gene is inserted into a suitable vector, typically a plasmid. Plasmids are circular DNA molecules capable of replicating within host cells, _______ ____ __________ _____ ________ ______ ________.
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