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Gene expression in prokaryotes is primarily regulated at the level of transcription, and operons provide a classic model to understand positive and negative regulation. An operon is a cluster of structural genes under the control of a single promoter and operator, transcribed together as a polycistronic mRNA. Regulatory proteins interact with specific DNA sequences to either inhibit or stimulate transcription. Negative regulation occurs when a _______ _____ ___ ______ ______ ____ _____.
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