Explain the principle of flame atomic absorption spectrometry. 5. Distinguish between various types of PCR techniques.
Flame Atomic Absorption Spectrometry (FAAS) is a widely used analytical technique in environmental monitoring for the detection and quantification of metal ions in various environmental samples such as water, soil, and air particulates. It is particularly valued for its simplicity, sensitivity, and precision when analyzing trace metals like lead (Pb), cadmium (Cd), copper (Cu), zinc (Zn), and iron (Fe).
Principle of Flame Atomic Absorption Spectrometry (FAAS):
The principle of FAAS is based on the absorption of radiation by free atoms in the ground state. The core concept revolves around the fact that when a solution containing metal ions is aspirated into a flame, the thermal energy of the flame converts the metal ions into free atoms. These atoms, now in the gaseous state, can absorb light of a specific wavelength that is characteristic of the particular element.
In a typical FAAS setup, a hollow cathode lamp is used as the radiation source. This lamp emits light of a specific wavelength corresponding to the element of interest. As the light passes through the flame containing the atomized sample, a portion of the light is absorbed ____ _________ __________ __________ ________ _________ ___.
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