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To isolate an enzyme from a crude protein extract using chromatographic techniques, a systematic purification strategy is essential. The process usually begins with selecting appropriate methods based on the enzyme’s properties such as size, charge, hydrophobicity, and affinity for specific ligands. First, start with ammonium sulfate precipitation to concentrate and partially purify the enzyme by exploiting its solubility differences. After precipitation and dialysis to remove salts, _________ ______ _____ _______ ____ ________.
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