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Fluorescence in situ hybridization (FISH) is a powerful bioanalytical technique used to detect and localize specific nucleic acid sequences within cells or tissue samples using fluorescently labeled probes. The first step in FISH is sample preparation, which involves fixing the cells or tissue on a slide and permeabilizing the membranes to allow probe access to ____ ______ ______ __________ __________ _________ ____ _______ ______ ____ _____ _________.
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