IGNOU BBCCT 125 SOLVED ASSIGNMENT
BBCCT 125: Genetic Engineering and Biotechnology
₹80 ₹30
| Title Name | IGNOU BBCCT 125 SOLVED ASSIGNMENT |
|---|---|
| Type | Soft Copy (E-Assignment) .pdf |
| University | IGNOU |
| Degree | BACHELOR DEGREE PROGRAMMES |
| Course Code | BSCBCH |
| Course Name | B.Sc. Honours in Biochemistry |
| Subject Code | BBCCT 125 |
| Subject Name | Genetic Engineering and Biotechnology |
| Year | 2026 |
| Session | - |
| Language | English Medium |
| Assignment Code | BBCCT 125/Assignment-1/2026 |
| Product Description | Assignment of BSCBCH (B.Sc. Honours in Biochemistry) 2026. Latest BBCCT 125 2026 Solved Assignment Solutions |
| Last Date of IGNOU Assignment Submission | Last Date of Submission of IGNOU BEGC-131 (BAG) 2025-26 Assignment is for January 2026 Session: 30th September, 2026 (for December 2025 Term End Exam). Semester Wise January 2025 Session: 30th March, 2026 (for June 2026 Term End Exam). July 2025 Session: 30th September, 2025 (for December 2025 Term End Exam). |
| Format | Ready-to-Print PDF (.soft copy) |
📅 Important Submission Dates
- January 2026 Session: 31st March, 2026
- July 2026 Session: 30th September, 2026
- January 2026 Session: 31st March, 2026
- July 2026 Session: 30th September, 2026
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• Guidelines: Strictly follows 2025-26 official word limits.
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BBCCT 125 2025 - English
ASSIGNMENT
MOLECULES OF LIFE
Course Code: BBCCT-125
Assignment Code: BBCCT-125/TMA/2025
Maximum Marks: 100
Answer all the questions given below.
1. Define Recombinant DNA Technology and describe the steps involved in its process.
2. What is the role of restriction endonucleases in genetic engineering? List and classify their types.
3. Compare and contrast Southern, Northern, and Western blotting techniques. What are their applications?
4. Explain the mechanism of action of DNA ligase in joining sticky and blunt-ended DNA fragments.
5. What are the steps involved in gene cloning, and why is a cloning vector necessary?
6. Explain the significance of synthetic oligonucleotides in molecular cloning and the steps involved in their synthesis.
7. Describe the polymerase chain reaction (PCR) and its variants. How does PCR contribute to genetic engineering?
8. Discuss the principles and methods used in DNA sequencing. Why is sequencing essential in genetic studies?
9. Illustrate the application of genetic engineering in medicine, focusing on the production of recombinant human insulin.
10. A) How has recombinant DNA technology influenced agricultural practices? Provide examples.
B) Discuss the environmental and ethical implications of genetically modified organisms (GMOs).
BBCCT 125 (January 2026 - July 2026) - ENGLISH
ASSIGNMENT
MOLECULES OF LIFE
Course Code: BBCCT-125
Assignment Code: BBCCT-125/TMA/2026
Maximum Marks: 100
Answer all the questions given below. All Questions carry equal marks.
1.
A) Describe the principle of recombinant DNA technology and list the major enzymes used in DNA manipulation.
B) Explain how restriction endonucleases and gel electrophoresis are used together to isolate and analyze a specific DNA fragment.
2.
A) Describe the essential features of an ideal cloning vector and explain the structure of pBR322.
B) Compare pUC and bacteriophage-based vectors and justify their use for cloning genes of different sizes.
3.
A) Describe the types of vectors used for gene cloning in yeast, plants, and animal cells.
B) Explain the choice of suitable eukaryotic vectors for expression of a human gene in yeast or plant systems.
4.
A) Explain the role of DNA ligase in joining DNA fragments and differentiate between sticky and blunt ends.
B) Describe how linkers, adapters, and synthetic oligonucleotides are used to clone blunt-ended DNA fragments.
5.
A) Describe the methods used for introduction of recombinant DNA into bacterial cells.
B) Explain the use of electroporation for DNA transfer into animal cells and its advantages over chemical methods.
6.
A) Explain the principles of insertional inactivation and blue-white selection.
B) Describe how recombinant phages are identified during bacteriophage-based cloning experiments.
7.
A) Describe the concept of gene libraries and the need for clone identification.
B) Explain how real-time PCR is used for quantitative analysis of gene expression.
8.
A) Describe the principle of Sanger's DNA sequencing method.
B) Explain the challenges in producing recombinant proteins in E. coli and the role of fusion tags in purification.
9.
A) Describe the principle of site-directed mutagenesis and outline the commonly used techniques.
B) Discuss the role of protein engineering in the development of biopharmaceuticals and industrial enzymes.
10.
A) Describe major applications of recombinant DNA technology in medicine and agriculture.
B) Discuss the production of recombinant insulin and analyze the safety concerns associated with genetically modified crops.
BBCCT 125 2025 - Hindi
सत्रीय कार्य
जीवन के अणु
पाठ्यक्रम कोड: BBCCT-125
सत्रीय कार्य कोड: BBCCT-125/TMA/2025
कुल अंक : 100
नीचे दिये गये सभी प्रश्नों के उत्तर दीजिये। सभी प्रश्नों के अंक समान है।
1. पुनः संयोजक डीएनए प्रौद्योगिकी को परिभाषित करें और इसकी प्रक्रिया में शामिल चरणों का वर्णन करें।
2. आनुवंशिक इंजीनियरिंग में प्रतिबंध एंडोन्यूक्लाइजेस की क्या भूमिका है? उनके प्रकारों की सूची बनाएं एवं उनका वर्गीकरण करें।
3. दक्षिणी, उत्तरी और पश्चिमी ब्लॉटिंग तकनीकों की तुलना करें और अंतर बताएं। उनके अनुप्रयोग क्या हैं?
4.चिपचिपे और कुंद सिरे वाले डीएनए टुकड़ों को जोड़ने में डीएनए लिगेज की क्रिया के तंत्र की व्याख्या करें।
5. जीन क्लोनिंग में क्या चरण शामिल हैं, और क्लोनिंग वेक्टर क्यों आवश्यक है?
6. आणविक क्लोनिंग में सिंथेटिक ऑलिगोन्यूक्लियोटाइड्स के महत्व और उनके संश्लेषण में शामिल चरणों की व्याख्या करें।
7. पोलीमरेज चेन रिएक्शन (पीसीआर) और इसके वेरिएंट का वर्णन करें। पीसीआर किस प्रकार योगदान देता है? जेनेटिक इंजीनियरिंग?
8. डीएनए अनुक्रमण में प्रयुक्त सिद्धांतों और विधियों पर चर्चा करें। अनुक्रमण क्यों आवश्यक है? आनुवंशिक अध्ययन?
9. के उत्पादन पर ध्यान केंद्रित करते हुए चिकित्सा में आनुवंशिक इंजीनियरिंग के अनुप्रयोग का वर्णन करें पुनः संयोजक मानव इंसुलिन.
10. ए) पुनः संयोजक डीएनए प्रौद्योगिकी ने कृषि पद्धतियों को कैसे प्रभावित किया है? उदाहरण प्रदान करें.
बी) आनुवंशिक रूप से संशोधित जीवों के पर्यावरणीय और नैतिक प्रभावों पर चर्चा करें (जीएमओ)
BBCCT 125 (January 2026 - July 2026) - HINDI
सत्रीय कार्य पुस्तिका
अनुवंशिक अभियांत्रिकी और जैवप्रौद्योगिकी
Course Code: BBCCT-125
Assignment Code: BBCCT-125/TMA/2026
Maximum Marks: 100
नीचे दिये गये सभी प्रश्नों के उत्तर दीजिये। सभी प्रश्नों के अंक समान है।
1. अ) पुनर्संयोजित डीएनए प्रौद्योगिकी के सिद्धांत का वर्णन कीजिए और डीएनए हेरफेर में प्रयुक्त प्रमुख एंजाइमों की सूची दीजिए। (5 अंक)
ब) यह समझाइए कि किसी विशिष्ट डीएनए खंड को पृथक करने और उसका विश्लेषण करने के लिए प्रतिबंध एंडोन्यूक्लिएज और जेल इलेक्ट्रोफोरेसिस का एक साथ उपयोग कैसे किया जाता है। (5 अंक)
2. अ) एक आदर्श क्लोनिंग वेक्टर की आवश्यक विशेषताओं का वर्णन कीजिए और pBR322 की संरचना की व्याख्या कीजिए। (5 अंक)
ब) pUC और बैक्टीरियोफेज-आधारित वेक्टरों की तुलना कीजिए और विभिन्न आकारों के जीनों की क्लोनिंग के लिए उनके उपयोग को उचित ठहराइए। (5 अंक)
3. अ) खमीर, पौधों और पशु कोशिकाओं में जीन क्लोनिंग के लिए उपयोग किए जाने वाले वेक्टरों के प्रकारों का वर्णन कीजिए। (5 अंक)
ब) खमीर या पादप प्रणालियों में मानव जीन की अभिव्यक्ति के लिए उपयुक्त यूकेरियोटिक वेक्टरों के चयन की व्याख्या कीजिए। (5 अंक)
4. अ) डीएनए खंडों को जोड़ने में डीएनए लाइगेज की भूमिका स्पष्ट कीजिए और चिपचिपे और कुंद सिरों के बीच अंतर बताइए। (5 अंक)
ब) कुंद सिरे वाले डीएनए खंडों को क्लोन करने के लिए लिंकर, एडेप्टर और सिंथेटिक ऑलिगोन्यूक्लियोटाइड का उपयोग कैसे किया जाता है, इसका वर्णन कीजिए। (5 अंक)
5. अ) जीवाणु कोशिकाओं में पुनर्संयोजित डीएनए को प्रवेश कराने के लिए उपयोग की जाने वाली विधियों का वर्णन कीजिए। 5 अंक
ब) पशु कोशिकाओं में डीएनए स्थानांतरण के लिए इलेक्ट्रोपोरेशन के उपयोग और रासायनिक विधियों पर इसके लाभों को स्पष्ट कीजिए। 5 अंक
6. अ) सम्मिलन निष्क्रियता और नीले–सफेद चयन के सिद्धांतों को स्पष्ट कीजिए। 5 अंक
ब) जीवाणु फेज–आधारित क्लोनिंग प्रयोगों के दौरान पुनर्संयोजित फेजों की पहचान कैसे की जाती है, इसका वर्णन कीजिए। 5 अंक
7. अ) जीन पुस्तकालयों की अवधारणा और क्लोन पहचान की आवश्यकता का वर्णन कीजिए। 5 अंक
ब) जीन अभिव्यक्ति के मात्रात्मक विश्लेषण के लिए वास्तविक समय पीसीआर का उपयोग कैसे किया जाता है, इसकी व्याख्या कीजिए। 5 अंक
8. अ) सैंगर की डीएनए अनुक्रमण विधि के सिद्धांत का वर्णन कीजिए। 5 अंक
ब) ई. कोलाई में पुनर्संयोजित प्रोटीन के उत्पादन में आने वाली चुनौतियों और शुद्धिकरण में संलयन टैग की भूमिका की व्याख्या कीजिए। 5 अंक
9. अ) साइट–निर्देशित उत्परिवर्तन के सिद्धांत का वर्णन कीजिए और आमतौर पर उपयोग की जाने वाली तकनीकों की रूपरेखा प्रस्तुत कीजिए। 5 अंक
ब) जैवऔषधीय पदार्थों और औद्योगिक एंजाइमों के विकास में प्रोटीन इंजीनियरिंग की भूमिका पर चर्चा कीजिए। 5 अंक
10. अ) चिकित्सा और कृषि में पुनर्संयोजित डीएनए प्रौद्योगिकी के प्रमुख अनुप्रयोगों का वर्णन कीजिए। 5 अंक
ब) पुनर्संयोजित इंसुलिन के उत्पादन पर चर्चा कीजिए और आनुवंशिक रूप से संशोधित फसलों से संबंधित सुरक्षा चिंताओं का विश्लेषण कीजिए। 5 अंक
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| Course Name | B.Sc. Honours in Biochemistry |
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| Language | English |
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