With the help of diagrams explain the steps of nuclear transplantation experiment in frog eggs.

Nuclear transplantation is a technique used to study cell differentiation and the potential of differentiated cells. In frog eggs, the process involves transferring the nucleus of one cell into an enucleated egg, with the goal of investigating whether the transplanted nucleus can direct the development of a new organism.

### Step 1: Egg Collection and Enucleation  
The first step involves the collection of unfertilized frog eggs. These eggs are then carefully enucleated, meaning their nuclei are removed using _______ _____ _______ ______ ______ _______ ______ ____ _____ __________ _____ ______.
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