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RNA splicing is a crucial post-transcriptional modification process in eukaryotic gene expression where non-coding sequences called introns are removed from the pre-messenger RNA (pre-mRNA) transcript, and coding sequences called exons are joined together to form a mature messenger RNA (mRNA) that can be translated into a functional protein. This process allows a single gene to give rise to multiple protein variants through alternative splicing, thereby increasing proteomic complexity without increasing the number of genes. RNA splicing is essential for proper gene expression, as the presence of introns in mature mRNA would lead to nonfunctional or truncated proteins. The splicing process is carried out by a large ribonucleoprotein complex called the spliceosome, which is composed of small ________ ____ ____ _______ ____.
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