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De novo cholesterol biosynthesis is tightly regulated at a specific rate-limiting step to maintain cellular cholesterol homeostasis. The key regulatory enzyme of this pathway is 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase). This enzyme catalyzes the conversion of HMG-CoA _______ __________ ___ ___ ________ ___ _________ _________ __________.
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