Question

Ion exchange and Affinity chromatography

27 Jan 2024
Answer :
Word Count : 287
AspectIon Exchange ChromatographyAffinity Chromatography
PrincipleSeparation based on the charge of the molecules. The stationary phase has charged groups that interact with charged biomolecules.Separation based on specific binding between a target molecule and a ligand (usually a protein or small molecule) on the column.
Target MoleculeCharged biomolecules like proteins, nucleic acids, or other charged substances.Specific proteins, enzymes, antibodies, or any molecule with a specific binding affinity to the ligand.
Separation MechanismBased on electrostatic interaction between ____ ________ ______ _________ ___ _____ _______.
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