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SDS-PAGE (Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis) is a widely used technique for separating proteins based on their molecular weight. In this method, proteins are first treated with SDS, an anionic detergent that denatures the proteins by disrupting non-covalent bonds, thereby linearizing them and imparting a uniform negative charge proportional to their length. This __________ _________ _______ __________ __________ _________ ______ ________ ________ _____.
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