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Fluorescence in situ hybridization (FISH) is a powerful cytogenetic technique used to detect and localize specific DNA sequences on chromosomes. The first step in the FISH procedure is sample preparation, which involves obtaining metaphase chromosome spreads from dividing cells or using interphase nuclei. The chromosomes are then fixed onto a glass slide and treated with protease or detergent to permeabilize the cells, making the DNA accessible for ________ ____ __________ _________ _________ _______ ________ ________ _________ _______ ____.
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