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Physical adsorption is the simplest and oldest method of enzyme immobilization. In this method, enzymes are attached to the surface of insoluble carriers or matrices by weak physical forces such as van der Waals forces, hydrogen bonds, hydrophobic interactions, ionic interactions, and electrostatic forces. No covalent bond is formed between the enzyme and the support material. The enzyme gets adsorbed on the carrier surface under suitable conditions of pH, temperature, and ionic strength. Various _____ _________ _______ _________ __________ _______ ____ __________.
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