Explain methodology of radioimmunoassay and list its advantages.

Question

23 Jan 2025

Answer :

Word Count : 389

Radioimmunoassay (RIA) is a sensitive technique used to measure the concentration of antigens, including hormones, drugs, and proteins, in biological samples. The methodology of RIA involves the principle of antigen-antibody interaction, where a radioactive isotope is used to label the antigen. The procedure consists of several key steps:

1. Antigen Preparation: A known amount of antigen is labeled with a radioactive isotope, usually iodine-125 or iodine-131. The labeled antigen is used to compete with the unlabeled antigen from the sample for binding to a specific antibody.

2. Antibody Preparation: A specific antibody is introduced into the reaction ____ _____ ___ ______ _________ ___ _____ ______ _________.
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