Discuss the technique of hypophysation in details.

Hypophysation is a reproductive technique used in freshwater aquaculture, primarily for the induced breeding of fish species. It involves the artificial induction of spawning in fish through the administration of hormones, specifically from the pituitary gland (hypophysis), which is responsible for the secretion of several key reproductive hormones. Hypophysation is particularly important for species that do not breed in captivity under natural conditions or those that have a prolonged breeding season. This technique is widely used for breeding carps, catfish, and other commercially important species in freshwater aquaculture.

The process of hypophysation typically involves three primary steps: collection of the hypophysis, hormone extraction, and injection into the broodfish. 

1. Collection of Pituitary Gland:
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