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Cholesterol biosynthesis is a tightly regulated metabolic pathway occurring mainly in the liver and, to a lesser extent, in the intestine and other tissues. Since cholesterol is essential for membrane structure, steroid hormone synthesis, and bile acid formation, but potentially harmful in excess, its production is controlled at multiple levels, with 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) serving as ____ _________ ___ ______ ___ ________ ________ ________.
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