Discuss the principle of coloumn chromatography illustrating the experimental setup.

Column chromatography is a widely used separation technique in chemistry, particularly in the purification of organic compounds. The principle of column chromatography is based on differential partitioning of components of a mixture between a stationary phase and a mobile phase.

Experimental Setup:

1. Column: A glass column with a stopcock at the bottom is used. The column is packed with a stationary phase, typically silica gel or alumina, which serves as the solid support for separation.

2. Stationary Phase: Silica gel or alumina is chosen based on the polarity of the compounds being separated. _________ _______ ______ ____ ________ ___ ______ __________ _____.
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