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Proteins are studied using a wide range of biochemical, biophysical, and molecular techniques to understand their structure, function, and interactions. Protein isolation and purification is usually the first step, commonly achieved through methods such as salting out, dialysis, and chromatography. Chromatographic techniques include ion-exchange chromatography, which separates proteins based on charge, gel filtration chromatography based on molecular size, and affinity ________ ____ ______ ____ _______ _____ __________ _________.
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