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Lambert-Beer’s law states that absorbance is directly proportional to the concentration of the absorbing species and the path length of the medium. However, in practical spectroscopic studies, deviations from this law are frequently observed due to various reasons. These deviations are generally classified into real, chemical, and instrumental factors. Real deviations arise when the fundamental assumptions of the law are not _______ ___ ______ _____ _____ ________.
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