Discuss methods used for evaluation of antimicrobial efficacy.

Evaluation of antimicrobial efficacy is essential in microbiology to determine the effectiveness of antimicrobial agents against specific microorganisms. Various methods are employed, broadly categorized into in vitro and in vivo techniques.

One of the most common in vitro methods is the disk diffusion method (Kirby-Bauer method). In this assay, antibiotic-impregnated disks ______ _______ _______ ____ ___ ___ ________ __________ ______.
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