Word Count : 244

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Edman degradation is a classical method for identifying the N-terminal amino acid of peptides under mild conditions without disturbing the remaining sequence. In this process, the peptide reacts with phenylisothiocyanate (PITC) in a slightly alkaline medium, similar to how nucleophilic addition reactions occur in Functional Group Organic Chemistry-I. ______ ______ __________ ____ ___ ______ _____.
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