Differentiate between transmission and scanning electron microscopes.

Question

27 Jan 2024

Answer :

Word Count : 568

Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM) are both powerful tools used in cell and molecular biology to visualize structures at the micro and nanometer scales. However, they operate on different principles and have distinct advantages and limitations.

Transmission Electron Microscope (TEM):

1. Principle of Operation:
- TEM operates on the principle of transmission of electrons through the specimen.
- A beam of electrons is transmitted through an ultra-thin section of the specimen.
- Interaction of electrons with the specimen generates an image.

2. Magnification and Resolution:
- TEM offers extremely high magnification, typically up to 1 million times.
- It provides ultra-high resolution, allowing visualization of subcellular structures and even individual molecules.
- The resolution of TEM can reach below 0.1 nanometers, enabling detailed examination of cellular ________ ________ _________ ___ ___.
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