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SDS-PAGE (Sodium Dodecyl Sulfate–Polyacrylamide Gel Electrophoresis) is a widely used bioanalytical technique for separating proteins based on their molecular weight. The technique combines the denaturing properties of SDS with the sieving effect of a polyacrylamide gel. SDS, an anionic detergent, binds uniformly to protein molecules, giving them ____ _________ ________ ____ ________.
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