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The effect of pH on enzyme activity is an important factor in determining the efficiency of enzymatic reactions because enzymes are proteins with specific three-dimensional structures that are sensitive to changes in the surrounding hydrogen ion concentration. Each enzyme has an optimum pH at which its activity is highest, and deviation from this optimum pH can lead to decreased activity or even complete denaturation. The pH affects enzyme activity primarily by altering the ionization state of amino acid _______ _________ ____ ______ _____ _________ ______ _______ _________ ________.
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