Question
Explain the principle of ammonium sulphate fractionation and dialysis in protein purification.
Answer :
Word Count : 206
Ammonium sulfate fractionation is a technique used to precipitate proteins based on their solubility. When ammonium sulfate is gradually added to a protein solution, the solubility of the proteins decreases as the ionic strength increases. Proteins with varying solubility at different ammonium sulfate concentrations can be separated. At a specific concentration, ____ ___ _______ ________ _________ ____.
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Ammonium sulfate fractionation is a technique used to precipitate proteins based on their solubility. When ammonium sulfate is gradually added to a protein solution, the solubility of the proteins decreases as the ionic strength increases. Proteins with varying solubility at different ammonium sulfate concentrations can be separated. At a specific concentration, ____ ___ _______ ________ _________ ____.
________ ____ _______ ______ _______ ________ ___.
_______ _________ __________ ________ _____.
______ ______ _________ _________ _________ ______ ___ ___ ______ ________ __________ _______.
________ ___ ___ _________ ______.
__________ __________ ______ _________ _________ _____.
___ _______ ____ ____ ____ ___ ___ ____ _____ __________ _________.
________ _________ ________ _______ ____ ________ ________ __________ ________ _______.
_________ ___ _____ ____ ___ ____.
________ ___ _____ _______ ________ ____ ________ _______ _______ _____ _______ ___.
____ ______ ____ ________ _________ __________ ________ ___.
______ _________ ________ __________ _____ ___ ___ _______ ____ ____ _____ ______.
_______ __________ _________ _____ ____.
__________ __________ ________ _____ _______ __________ _______.
____ ___ ______ _______ __________ ___.
____ _______ ______ ___ _____ ___ _______ __________ ___ ____ _______ _____.
________ _________ ______ ____ ______ __________ _________ ___ ________ ________ __________.
_________ ____ ___ ___ ___ ______.
______ ________ __________ ______ _________.
_________ __________ ________.
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