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Bioleaching is a process within environmental biotechnology that involves the extraction of metals from their ores through the use of living microorganisms. This method offers an eco-friendly and cost-effective alternative to traditional metallurgical techniques such as smelting and chemical leaching, which often involve high energy consumption and the release of toxic by-products. In bioleaching, certain microorganisms, primarily bacteria and fungi, are employed to solubilize metals from ores, enabling their recovery from low-grade mineral resources that would otherwise be economically unfeasible to exploit. The process has been widely applied for the recovery of metals such as copper, gold, nickel, uranium, and zinc. The bioleaching process can occur through two primary mechanisms: direct and indirect bioleaching. In direct bioleaching, microorganisms attach themselves to the surface of the mineral ore and directly oxidize the metal compounds. For instance, in the bioleaching of sulfide ores, bacteria such as *Acidithiobacillus ferrooxidans* and *Leptospirillum ferrooxidans* oxidize ferrous ions (Fe²⁺) to ferric ions (Fe³⁺), which then react with the sulfide minerals to release soluble metal ions. Direct contact between the bacteria and mineral surface enhances the efficiency of metal solubilization. On the other hand, indirect bioleaching does not require direct contact between the microorganism and the ore. Instead, the microorganisms oxidize an intermediate compound, such as iron or sulfur, to generate an oxidizing agent that chemically interacts with the mineral to release the metal. __________ ___ _______ ____ _______.
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