Explain sanger method for the N-terminal identification of peptides giving suitable reactions.

The Sanger method, also known as the Sanger’s dinitrofluorobenzene (DNFB) method, is a technique used to identify the N-terminal amino acid of peptides. It involves the reaction of a peptide with a reagent, 1-fluoro-2,4-dinitrobenzene (DNFB), which reacts with the free amino group of the N-terminal amino acid.

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