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Biogas generation is a biological process in which microorganisms decompose organic matter under anaerobic conditions to produce methane, carbon dioxide, and small amounts of other gases. The efficiency of this process depends on several environmental and operational parameters, among which temperature, pH, and carbon-to-nitrogen (C/N) ratio play a crucial role. These parameters directly influence microbial activity, digestion rate, and overall gas yield. Temperature is one of the most important factors affecting biogas production because the microorganisms involved in anaerobic digestion are highly sensitive to temperature changes. Biogas generation typically occurs under two temperature ranges: mesophilic conditions (around 30–40°C) and thermophilic conditions (around 50–60°C). Under mesophilic conditions, microbial __________ __________ ___ ____ ___ ___.
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