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Solvent fractionation and lyophilization are key techniques used in protein purification to concentrate and stabilize proteins, each playing a distinct role in the process. Solvent fractionation involves the addition of solvents such as ammonium sulfate or acetone to a protein solution, which causes proteins to precipitate out of solution based on their solubility. By adjusting the solvent concentration, proteins ______ ______ _________ _____ _________ ___ _________ _______ _____ ______.
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