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Cloning blunt-ended DNA fragments often requires specialized techniques because blunt ends lack the sticky overhangs that facilitate easy ligation. Linkers, adapters, and synthetic oligonucleotides are molecular tools used to modify blunt-ended DNA fragments to enable their cloning. Linkers are short, double-stranded DNA sequences with a known restriction enzyme recognition site. When blunt-ended fragments are ___ _________ ____ ____ _________ ____ __________.
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