Define denaturation of DNA and explain the effect of temperature and pH on DNA.

Denaturation of DNA refers to the process in which the double-helix structure of the DNA molecule unwinds and separates into two single strands. This disruption in the structure occurs when the hydrogen bonds between the complementary base pairs (adenine-thymine and guanine-cytosine) are broken, leading to the ____ ___ ____ ____ ____ ___ _____ ________ _______.
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